Composite

Part:BBa_K5441010:Design

Designed by: Lok Yin Wong   Group: iGEM24_PLKLFC   (2024-10-01)


PSMA_GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1995
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 630
    Illegal BsaI.rc site found at 1917


Design Notes

As the original PSMA promoter had several illegal restriction sites as defined by iGEM, some bases were changed slightly to prevent those sites from forming. Experiments were conducted using this modified PSMA promoter and fluorescence was still detected.


Source

PSMA promoter: AF007544.1 GFP (Aequorea victoria green fluorescent protein): original wild-type sequence

References